Additional Info:
Includes gliadin antibody IgG and gliadin antibody IgA.
Useful For:
Gliadin (Deamidated Peptide) Antibody (IgG, IgA) – Detection of antibodies to gliadin, one of the major protein components of gluten, is a sensitive assay useful in diagnosing celiac disease. However, gliadin antibodies may be found in individuals without celiac disease; thus gliadin antibody assays are less specific than assays measuring antibodies to endomysium and transglutaminase. Recent work has revealed that gliadin-reactive antibodies from celiac patients bind to a very limited number of specific epitopes on the gliadin molecule. Further, deamidation of gliadin results in enhanced binding of gliadin antibodies. Based on this information, assays using deamidated gliadin peptides bearing the celiac-specific epitopes have much higher diagnostic accuracy for celiac disease when compared to standard gliadin antibody assays.
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Specimen Requirements:
1 yellow-top SST tube (minimum: 0.5 mL of serum). Centrifuge SST 30 minutes after draw. Refrigerate specimen after collection.
Analytical Time: No turnaround times
Days Set Up: No setup days
CPT Information:
83516 – X 2 Gliadin IgA & IgG