Epstein-Barr Virus (EV) Nuclear Antigen, IgG Antibody, Serum

Useful For: The Epstein-Barr Virus (EBV) is the causative agent of infectious mononucleosis (IM). IM is an acute illness characterized clinically by sore throat, fever, and lymphadenopathy. In children, 5-10% of primary Epstein-Barr Virus (EBV) infections are asymptomatic. By adulthood, 90-95% of most populations have demonstrable EBV antibodies.

EBV-specific serodiagnostic tests are not usually needed in typical cases of IM since 90% of the cases are heterophile-positive. For heterophile-negative cases and for diagnosis in atypical cases, confirmation of infection by EBV-specific serology is useful. EBNA anti-bodies arise from 3 weeks to several months after the onset of the illness and can confirm diagnosis. Previous EBV infections are identified by IgG antibodies to VCA and to EBNA.

Methodology: Enzyme Immuno Assay (EIA)

Specimen Requirements: 1 yellow-top SST tube (minimum: 0.5 mL of serum). Centrifuge SST 30 minutes after draw. Refrigerate specimen after collection.

NOTE:
Fresh sample is preferred.

Reject Criteria
Gross hemolysis • Gross lipemia • Icteric

Analytical Time: 2 days

Days Set Up: Monday through Friday

CPT Information:
86664 –

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