Useful For:
General screen for carrier status and assessment of CF risk. This test will identify approximately 90% of Cystic Fibrosis (CF) mutations in the Caucasian population, and 97% in the Ashkenazi-Jewish population.
Methodology: PCR and Oligonucleotide Ligation Assay
Specimen Requirements: 2 lavender-top (EDTA) tubes (minimum: 3.0 mL of EDTA whole blood). Store specimen at room temperature after collection. Specimen must be sent immediately to the lab.
NOTE:
Complete “Cystic Fibrosis Screen” history sheet available from Meadville Medical Center Laboratory and send with patient or specimen. This from also may be found in “Forms-History/Patient Information” section of this manual. Include patient history, clinical information, reason for referral, and referring physician’s name and phone number.
2 mL of saliva in Oragene DNA Self-Collection kit is acceptable.
For genetic testing, original tube is required.
Yellow-top (ACD-A or ACD-B) are also acceptable.
Analytical Time: 8 days
Days Set Up: Monday through Sunday
CPT Information:
83891 – Molec dx, isol or Extract of nucl acd
83900 – Molec dx, amp, tgt, multi 1st 2 seq.
83901 – x 13 Molec dx, amp, tgt multi addl seq
83909 – Molec dx, sep and ID by hi res
83912 – Molec dx; interp and report
83914 – x 23 Mutation ID-enzym lig. or prim ext, each